HDL Cholesterol

HDL Cholesterol

In the first step of the reaction, Anti Human lipoprotein antibody binds to lipoproteins other than HDL. The complexes thus formed inhibit enzyme reactions when the reagent 2 is added. Cholesterol Oxidsase and Cholesterol Esterase in the R2 react only with HDL C. Hydrogen Peroxide produced by the enzyme reaction with HDL C produces a blue colour complex upon oxidative condensation of F-DOAS and 4 Amino Antipyrine in the presence of Peroxidase. By measuring the absorbance of the Blue coloured complex, HDL C concentration is quantitated.

Fortress Diagnostics manufactures HDL reagents designed for Hitachi 917 and Olympus AU600/400/640/2700.